The Simply Seamless technology was developed to improve the speed, efficiency and accuracy of DNA assembly and cloning. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with 25 bp overlaps. Ideal for the Synthetic Biology community, Simply Seamless makes one-step cloning of multiple fragments easy, fast, and reliable. The reaction includes a proprietary mix of enzymes that work together in the same buffer.
a) Diagram
illustrating the assembly of four 1 kb fragments containing 25 bp homologous overlap regions.
b) 1% Agarose gel comparing the two assembly reactions: 1. Simply Seamless..
100 ng of each PCR fragment was assembled in each reaction. The 4kb band is the fully assembled product.
a) 1% Agarose gel comparing the two assembly reactions: 1. Simply Seamless, 2. Competitor. 100 ng of a PCR fragment (3.8 kb) was cloned into a vector (2.9 kb) in each reaction. The 6.82 kb band is the fully assembled product.
b) Graph comparing the number of transformants (CFUs/ml) from the two different reactions. 3 ul of each 10 ul reaction was transformed and plated on LB with ampicillin
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Enjoy our huge reductions on bulk purchases. The Simply Seamless kit is shipped in dry ice.
You can order by email at order.kit@synovance.com or online.
For larger quantities, contact us directly by mail at : info@synovance.com
